goat polyclonal antibody against collagen iii  (ProSci Incorporated)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Quantitative Stain-Free Conjunctival Collagen Imaging in Cicatrizing Conjunctivitis Using Second Harmonic Generation-Two Photon Excitation Technology

doi: 10.1167/iovs.66.4.49

Figure Lengend Snippet: Immunohistochemistry and unstained SHG images of conjunctival tissues from healthy controls participants and patients with chronic ocular SJS. Left and middle panels showing representative raw immunohistology and corresponding processed grayscale images highlighting collagen Type 1 and Type 3 in the conjunctiva of control and diseased subjects; the green represents antibody positive signals and blue represents cell nuclei; Right panels show representative SHG images of the same control patient and diseased patients; red demarcation line represents the region of interest; bar graph showing higher collagen III / I ratio in diseased tissues compared to normal controls.

Article Snippet: Sections on the slides were initially washed in 1× PBS (1st BASE), permeabilized with 0.15% Triton X-100 (Sigma-Aldrich) for 30 minutes, blocked with 2% bovine serum albumin (Sigma-Aldrich) and 5% normal goat serum (Thermo Fisher Scientific) for one hour, and double-stained with a mouse monoclonal antibody against collagen type I (Sigma-Aldrich) and a goat polyclonal antibody against collagen type III (Southern Biotech, Birmingham, AL, USA) at 4°C overnight.

Techniques: Immunohistochemistry, Control